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non tissue culture treated roundbottom 96 well plates  (CELLTREAT Scientific)


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    CELLTREAT Scientific non tissue culture treated roundbottom 96 well plates
    Non Tissue Culture Treated Roundbottom 96 Well Plates, supplied by CELLTREAT Scientific, used in various techniques. Bioz Stars score: 94/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/non+tissue+culture+treated+roundbottom+96+well+plates/Non-treated+Multiple+Well+Plates/pm34630389-84-21-27
    Average 94 stars, based on 13 article reviews
    non tissue culture treated roundbottom 96 well plates - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Ex Vivo:

    Article Title: Lymph Node-Targeted Synthetically Glycosylated Antigen Leads to Antigen-Specific Immunological Tolerance.
    Article Snippet: Antibodies against the following markers were used: CD3 – BUV395 (BD Biosciences 563565), CD8-BUV737 (BD Biosciences 612759), CD4 – BUV496 (BD Biosciences 612952), Foxp3 – FITC (BD Biosciences 560403), CD25 – BV605 (BioLegend 120235), ST2 – BV421 (BD Biosciences 566309), Lag3 – PerCP-Cy5.5 (BD Biosciences 564673), CTLA4 – PE-Cy7 (eBioScience 17-1522- 82), IFNg – APC (BioLegend 505810), TNFa – BV605 (BioLegend 506329), IL-2 – FITC (BioLegend 503806), IL-10 – APC-Cy7 (BioLegend 505036), PD-1 – BV711 (BioLegend 135231), Tim3 – PE (BD Biosciences 566346). .. Ex Vivo Antigen-Specific Restimulation LN and spleen single-cell suspensions were seeded at a count of 1x106 - 3x106 per well in non-tissue culture treated roundbottom 96-well plates (Celltreat 229590), and stimulated ex vivo at 37°C for 2 h with either OVA257-264 peptide (Genscript) at a final concentration of 1 mg/mL, or OVA323-339 peptide (Genscript) at 2 mg/mL, followed by Brefeldin A at a final concentration of 5 mg/mL for another 4 h. The cells were then washed with PBS before proceeding with cytokine antibody staining for flow cytometry. .. For long-term restimulations, grade V OVA (Sigma A5503) was added to cells at a final concentration of 100 mg/mL for 4 days.

    Single Cell:

    Article Title: Lymph Node-Targeted Synthetically Glycosylated Antigen Leads to Antigen-Specific Immunological Tolerance.
    Article Snippet: Antibodies against the following markers were used: CD3 – BUV395 (BD Biosciences 563565), CD8-BUV737 (BD Biosciences 612759), CD4 – BUV496 (BD Biosciences 612952), Foxp3 – FITC (BD Biosciences 560403), CD25 – BV605 (BioLegend 120235), ST2 – BV421 (BD Biosciences 566309), Lag3 – PerCP-Cy5.5 (BD Biosciences 564673), CTLA4 – PE-Cy7 (eBioScience 17-1522- 82), IFNg – APC (BioLegend 505810), TNFa – BV605 (BioLegend 506329), IL-2 – FITC (BioLegend 503806), IL-10 – APC-Cy7 (BioLegend 505036), PD-1 – BV711 (BioLegend 135231), Tim3 – PE (BD Biosciences 566346). .. Ex Vivo Antigen-Specific Restimulation LN and spleen single-cell suspensions were seeded at a count of 1x106 - 3x106 per well in non-tissue culture treated roundbottom 96-well plates (Celltreat 229590), and stimulated ex vivo at 37°C for 2 h with either OVA257-264 peptide (Genscript) at a final concentration of 1 mg/mL, or OVA323-339 peptide (Genscript) at 2 mg/mL, followed by Brefeldin A at a final concentration of 5 mg/mL for another 4 h. The cells were then washed with PBS before proceeding with cytokine antibody staining for flow cytometry. .. For long-term restimulations, grade V OVA (Sigma A5503) was added to cells at a final concentration of 100 mg/mL for 4 days.

    Concentration Assay:

    Article Title: Lymph Node-Targeted Synthetically Glycosylated Antigen Leads to Antigen-Specific Immunological Tolerance.
    Article Snippet: Antibodies against the following markers were used: CD3 – BUV395 (BD Biosciences 563565), CD8-BUV737 (BD Biosciences 612759), CD4 – BUV496 (BD Biosciences 612952), Foxp3 – FITC (BD Biosciences 560403), CD25 – BV605 (BioLegend 120235), ST2 – BV421 (BD Biosciences 566309), Lag3 – PerCP-Cy5.5 (BD Biosciences 564673), CTLA4 – PE-Cy7 (eBioScience 17-1522- 82), IFNg – APC (BioLegend 505810), TNFa – BV605 (BioLegend 506329), IL-2 – FITC (BioLegend 503806), IL-10 – APC-Cy7 (BioLegend 505036), PD-1 – BV711 (BioLegend 135231), Tim3 – PE (BD Biosciences 566346). .. Ex Vivo Antigen-Specific Restimulation LN and spleen single-cell suspensions were seeded at a count of 1x106 - 3x106 per well in non-tissue culture treated roundbottom 96-well plates (Celltreat 229590), and stimulated ex vivo at 37°C for 2 h with either OVA257-264 peptide (Genscript) at a final concentration of 1 mg/mL, or OVA323-339 peptide (Genscript) at 2 mg/mL, followed by Brefeldin A at a final concentration of 5 mg/mL for another 4 h. The cells were then washed with PBS before proceeding with cytokine antibody staining for flow cytometry. .. For long-term restimulations, grade V OVA (Sigma A5503) was added to cells at a final concentration of 100 mg/mL for 4 days.

    Staining:

    Article Title: Lymph Node-Targeted Synthetically Glycosylated Antigen Leads to Antigen-Specific Immunological Tolerance.
    Article Snippet: Antibodies against the following markers were used: CD3 – BUV395 (BD Biosciences 563565), CD8-BUV737 (BD Biosciences 612759), CD4 – BUV496 (BD Biosciences 612952), Foxp3 – FITC (BD Biosciences 560403), CD25 – BV605 (BioLegend 120235), ST2 – BV421 (BD Biosciences 566309), Lag3 – PerCP-Cy5.5 (BD Biosciences 564673), CTLA4 – PE-Cy7 (eBioScience 17-1522- 82), IFNg – APC (BioLegend 505810), TNFa – BV605 (BioLegend 506329), IL-2 – FITC (BioLegend 503806), IL-10 – APC-Cy7 (BioLegend 505036), PD-1 – BV711 (BioLegend 135231), Tim3 – PE (BD Biosciences 566346). .. Ex Vivo Antigen-Specific Restimulation LN and spleen single-cell suspensions were seeded at a count of 1x106 - 3x106 per well in non-tissue culture treated roundbottom 96-well plates (Celltreat 229590), and stimulated ex vivo at 37°C for 2 h with either OVA257-264 peptide (Genscript) at a final concentration of 1 mg/mL, or OVA323-339 peptide (Genscript) at 2 mg/mL, followed by Brefeldin A at a final concentration of 5 mg/mL for another 4 h. The cells were then washed with PBS before proceeding with cytokine antibody staining for flow cytometry. .. For long-term restimulations, grade V OVA (Sigma A5503) was added to cells at a final concentration of 100 mg/mL for 4 days.

    Flow Cytometry:

    Article Title: Lymph Node-Targeted Synthetically Glycosylated Antigen Leads to Antigen-Specific Immunological Tolerance.
    Article Snippet: Antibodies against the following markers were used: CD3 – BUV395 (BD Biosciences 563565), CD8-BUV737 (BD Biosciences 612759), CD4 – BUV496 (BD Biosciences 612952), Foxp3 – FITC (BD Biosciences 560403), CD25 – BV605 (BioLegend 120235), ST2 – BV421 (BD Biosciences 566309), Lag3 – PerCP-Cy5.5 (BD Biosciences 564673), CTLA4 – PE-Cy7 (eBioScience 17-1522- 82), IFNg – APC (BioLegend 505810), TNFa – BV605 (BioLegend 506329), IL-2 – FITC (BioLegend 503806), IL-10 – APC-Cy7 (BioLegend 505036), PD-1 – BV711 (BioLegend 135231), Tim3 – PE (BD Biosciences 566346). .. Ex Vivo Antigen-Specific Restimulation LN and spleen single-cell suspensions were seeded at a count of 1x106 - 3x106 per well in non-tissue culture treated roundbottom 96-well plates (Celltreat 229590), and stimulated ex vivo at 37°C for 2 h with either OVA257-264 peptide (Genscript) at a final concentration of 1 mg/mL, or OVA323-339 peptide (Genscript) at 2 mg/mL, followed by Brefeldin A at a final concentration of 5 mg/mL for another 4 h. The cells were then washed with PBS before proceeding with cytokine antibody staining for flow cytometry. .. For long-term restimulations, grade V OVA (Sigma A5503) was added to cells at a final concentration of 100 mg/mL for 4 days.



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    non tissue culture treated roundbottom 96 well plates  (CELLTREAT Scientific)
    94
    CELLTREAT Scientific non tissue culture treated roundbottom 96 well plates
    Non Tissue Culture Treated Roundbottom 96 Well Plates, supplied by CELLTREAT Scientific, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/non+tissue+culture+treated+roundbottom+96+well+plates/Non-treated+Multiple+Well+Plates/pm34630389-84-21-27
    Average 94 stars, based on 1 article reviews
    non tissue culture treated roundbottom 96 well plates - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier

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